| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 681 | Clostridium butyricum | AGGCATGCTAAAGGGCAAC | ATGCCACCAGCTGCACTT | 58.42 | 59.88 | 276 | 75 |
100.00%
|
50.00%
|
| Primer 682 | Clostridium butyricum | GCATGCTAAAGGGCAACACT | ATGCCACCAGCTGCACTT | 59.11 | 59.88 | 274 | 75 |
100.00%
|
50.00%
|
| Primer 683 | Clostridium butyricum | TAGGCATGCTAAAGGGCAACA | ATGCCACCAGCTGCACTT | 59.99 | 59.88 | 277 | 75 |
100.00%
|
50.00%
|
| Primer 684 | Clostridium butyricum | TGACCTTGCAGATGCCCA | AGCAGTGTGGAATGCCCA | 58.83 | 59.15 | 286 | 75 |
100.00%
|
50.00%
|
| Primer 685 | Clostridium butyricum | ACCTTGCAGATGCCCACA | TAGCTGAGCAGGGTCACCA | 59.15 | 60.23 | 185 | 75 |
100.00%
|
50.00%
|
| Primer 686 | Clostridium butyricum | ACCTTGCAGATGCCCACA | GAGCAGTGTGGAATGCCCA | 59.15 | 60.30 | 285 | 75 |
100.00%
|
50.00%
|
| Primer 687 | Clostridium butyricum | GACCTTGCAGATGCCCACA | AGCAGTGTGGAATGCCCA | 60.30 | 59.15 | 285 | 75 |
100.00%
|
50.00%
|
| Primer 688 | Clostridium butyricum | CCAGCATCTGCAAAAGTAGGG | ATCCCGTTGGAGGCAACA | 59.25 | 58.83 | 214 | 75 |
100.00%
|
50.00%
|
| Primer 689 | Clostridium butyricum | CCAGCATCTGCAAAAGTAGGG | TCCCGTTGGAGGCAACAAT | 59.25 | 59.54 | 213 | 75 |
100.00%
|
50.00%
|
| Primer 690 | Clostridium butyricum | CCAGCATCTGCAAAAGTAGGG | ATCCCGTTGGAGGCAACAA | 59.25 | 59.54 | 214 | 75 |
100.00%
|
50.00%
|